製品の概要
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製品名Anti-PSMA antibody [GCP-05]
PSMA 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [GCP-05] to PSMA
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由来種Mouse
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アプリケーション適用あり: IP, ICC, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA
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エピトープThis antibody recognizes the extracellular domain of PSMA.
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ポジティブ･コントロール
- LNCaP cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 15mM Sodium Azide
Constituents: PBS, pH ~7.4 -
Concentration information loading... -
精製度Protein A purified
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特記事項（精製）Purity: > 95% Purified from TCS
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ポリ/モノモノクローナル
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クローン名GCP-05
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アイソタイプIgG1
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab66912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
組織特異性Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.
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配列類似性Belongs to the peptidase M28 family. M28B subfamily.
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ドメインThe NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal.
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翻訳後修飾The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
細胞内局在Cytoplasm and Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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別名
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
画像
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![Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)](http://a.abcam.com/ps/products/66/ab66912/Images/ab66912-1.jpg)
Flow Cytometry - Anti-PSMA antibody [GCP-05] (ab66912)This image shows LNCaP cell line stained by ab66911 and ab66912. Green column - antibodies at 1µg/ml; Blue column - antibodies at 5µg/ml; Yellow column - negative cell line.
プロトコール
参考文献
This product has been referenced in:
- Castanares MA et al. Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. Prostate 76:215-25 (2016). Read more (PubMed: 26499105) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"