Anti-PSMA 抗体 [GCP-05] (ab66912)
Key features and details
- Mouse monoclonal [GCP-05] to PSMA
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-PSMA antibody [GCP-05]
PSMA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GCP-05] to PSMA -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA
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エピトープ
This antibody recognizes the extracellular domain of PSMA. -
ポジティブ・コントロール
- LNCaP cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purity: > 95% Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
GCP-05 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
組織特異性
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
配列類似性
Belongs to the peptidase M28 family. M28B subfamily. -
ドメイン
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
翻訳後修飾
The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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別名
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
画像
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Flow cytometry analysis of PSMA positive LNCaP cells stained with ab66912 (green) and PSMA negative HeLa cells (blue)
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This image shows LNCaP cell line stained by ab66911 and ab66912. Green column - antibodies at 1µg/ml; Blue column - antibodies at 5µg/ml; Yellow column - negative cell line.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab66912 は 8 報の論文で使用されています。
- Sharma S et al. Use of Glycoproteins-Prostate-Specific Membrane Antigen and Galectin-3 as Primary Tumor Markers and Therapeutic Targets in the Management of Metastatic Prostate Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35681683
- Kawakami K et al. Diagnostic potential of serum extracellular vesicles expressing prostate-specific membrane antigen in urologic malignancies. Sci Rep 11:15000 (2021). PubMed: 34294841
- Shirazi HS et al. Plasma enabled devices for the selective capture and photodynamic identification of prostate cancer cells. Biointerphases 15:031002 (2020). PubMed: 32414237
- Dostalova S et al. Prostate-Specific Membrane Antigen-Targeted Site-Directed Antibody-Conjugated Apoferritin Nanovehicle Favorably Influences In Vivo Side Effects of Doxorubicin. Sci Rep 8:8867 (2018). PubMed: 29891921
- Banerjee SR et al. 111In- and IRDye800CW-Labeled PLA-PEG Nanoparticle for Imaging Prostate-Specific Membrane Antigen-Expressing Tissues. Biomacromolecules 18:201-209 (2017). PubMed: 28001364
- Castanares MA et al. Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. Prostate 76:215-25 (2016). PubMed: 26499105
- Tykvart J et al. Comparative analysis of monoclonal antibodies against prostate-specific membrane antigen (PSMA). Prostate 74:1674-90 (2014). PubMed: 25262926
- Chen Z et al. PSMA-targeted theranostic nanoplex for prostate cancer therapy. ACS Nano 6:7752-7762 (2012). PubMed: 22866897