製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PSIP1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: TEQQNKDEGK KPEVKKVEKK RETSMDSRLQ RIHAEIKNSL KIDNLDVNRC, corresponding to internal sequence amino acids 324-373 of Human PSIP1

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

ターゲット情報

  • 機能
    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • 組織特異性
    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • 関連疾患
    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • 配列類似性
    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • ドメイン
    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLL associated antigen KW 7 antibody
    • CLL-associated antigen KW-7 antibody
    • Dense fine speckles 70 kDa protein antibody
    • DFS 70 antibody
    • DFS70 antibody
    • LEDGF antibody
    • Lens epithelium derived growth factor antibody
    • Lens epithelium-derived growth factor antibody
    • MGC74712 antibody
    • p52 antibody
    • p75 antibody
    • PAIP antibody
    • PC4 and SFRS1 interacting protein 1 antibody
    • PC4 and SFRS1 interacting protein 2 antibody
    • PC4 and SFRS1 interacting protein antibody
    • PC4 and SFRS1-interacting protein antibody
    • PSIP 1 antibody
    • PSIP 2 antibody
    • Psip1 antibody
    • PSIP1_HUMAN antibody
    • PSIP2 antibody
    • Transcriptional coactivator p75/p52 antibody
    see all

画像



  • Predicted band size : 60 kDa
    Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.


  • Predicted band size : 60 kDa
    Western blot analysis of Jurkat cell lysate labeling PSIP1 with ab49281 at 1µg/ml.
  • Anti-PSIP1 antibody (ab49281) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Thank you very much for your interest in ab49281. To our knowledge, ab49281 has not been tested using a Biacore. It is not possible to submit Abreviews for this application but we would be interested in your experience with IP. Therefore, I can...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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