製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PSIP1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human PSIP1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O75475

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Thymus and Human Brain tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

ターゲット情報

  • 機能
    Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
  • 組織特異性
    Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
  • 関連疾患
    Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • 配列類似性
    Belongs to the HDGF family.
    Contains 1 PWWP domain.
  • ドメイン
    Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Remains chromatin-associated throughout the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CLL associated antigen KW 7 antibody
    • CLL-associated antigen KW-7 antibody
    • Dense fine speckles 70 kDa protein antibody
    • DFS 70 antibody
    • DFS70 antibody
    • LEDGF antibody
    • Lens epithelium derived growth factor antibody
    • Lens epithelium-derived growth factor antibody
    • MGC74712 antibody
    • p52 antibody
    • p75 antibody
    • PAIP antibody
    • PC4 and SFRS1 interacting protein 1 antibody
    • PC4 and SFRS1 interacting protein 2 antibody
    • PC4 and SFRS1 interacting protein antibody
    • PC4 and SFRS1-interacting protein antibody
    • PSIP 1 antibody
    • PSIP 2 antibody
    • Psip1 antibody
    • PSIP1_HUMAN antibody
    • PSIP2 antibody
    • Transcriptional coactivator p75/p52 antibody
    see all

画像

  • All lanes : Anti-PSIP1 antibody (ab124667) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 115 kDa (possible non-specific binding),46 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    Exposure time : 12 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124667 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

プロトコール

参考文献

ab124667 has not yet been referenced specifically in any publications.

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