製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to PSF2
  • 由来種
    Chicken
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length native protein (purified) (Human) PSF2

  • ポジティブ・コントロール
    • E coli derived fusion protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Azide free.
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 21 kDa. We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
ICC/IF Use at an assay dependent dilution. PubMed: 20571081

ターゲット情報

  • 機能
    The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
  • 配列類似性
    Belongs to the GINS2/PSF2 family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA replication complex GINS protein PSF2 antibody
    • GINS complex subunit 2 (Psf2 homolog) antibody
    • GINS complex subunit 2 antibody
    • gins2 antibody
    • HSPC037 antibody
    • Pfs2 antibody
    • PSF2 antibody
    • PSF2_HUMAN antibody
    see all

画像

  • Anti-PSF2 antibody (ab37683) at 1/2000 dilution + E coli derived fusion protein

    Secondary
    Goat anti IgY-HRP at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 72 kDa (why is the actual band size different from the predicted?)



    A fusion protien has been used in this western blot, which accounts for the differences in molecular weight, compared to the native protien.

参考文献

This product has been referenced in:
  • Tumini E  et al. Physical and functional crosstalk between Fanconi anemia core components and the GINS replication complex. DNA Repair (Amst) 10:149-58 (2011). WB ; Human . Read more (PubMed: 21109493) »
  • Cohen SM  et al. BRG1 co-localizes with DNA replication factors and is required for efficient replication fork progression. Nucleic Acids Res 38:6906-19 (2010). ICC/IF ; Mouse . Read more (PubMed: 20571081) »

See all 3 Publications for this product

レビューと Q&A

Thank you for your inquiry; the source of this antibody traditionally uses the fusion proteins as testing positive controls, therefore we do not know how the antibody performs on endogenous protein I'm afraid.I can confirm that they have loaded between...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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