The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA titer: 1/5000.
ELISA titer is defined as the dilution of antibody (1mg/ml) sufficient to give a change in absorbance of 1.0 at 450nm.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Pseudomonas aeruginosa is Gram-negative, aerobic, rod-shaped bacteria with unipolar motility. An opportunistic human pathogen, P. aeruginosa is also an opportunistic pathogen of plants. P. aeruginosa bacteria are clinically important because they are resistant to most antibiotics and they are capable of surviving in conditions that few other organisms can tolerate. Pseudomonas is often encountered in hospital and clinical work because it is a major cause of hospital acquired (nosocomal) infections. Its main targets are immunocompromised individuals, burn victims, and individuals on respirators or with indwelling catheters. Additionally, these pathogens colonize the lungs of cystic fibrosis patients.
P. aeruginosa is often identified by its pearlescent appearance and grape-like odor in vitro. Definitive clinical identification of P. aeruginosa includes identifying the production of both pyocyanin and fluorescein as well as its ability to grow at 42°C. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as hydrocarbon utilizing microorganisms (or "HUM bugs"), causing microbial corrosion. It creates dark gellish mats sometimes improperly called "algae".
Chung CY et al. Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform. Sci Rep7:46336 (2017).
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