Anti-PSD95 (phospho T19) 抗体 (ab16496)

製品の概要

  • 製品名Anti-PSD95 (phospho T19) antibody
    PSD95 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PSD95 (phospho T19)
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PSD95, phosphorylated at T19.

    (Peptide available as ab17167.)

  • ポジティブ・コントロール
    • mouse forebrain
  • 特記事項


    Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Phosphorylation of PSD95 dynamically regulates the clustering of PSD95/NMDA receptors at synapses, providing a possible mechanism for rapid changes in density and/or number of receptor at synapses.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab16496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 組織特異性Brain.
  • 配列類似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • ドメインThe PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻訳後修飾Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 細胞内局在Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 (phospho T19) antibody 画像

  • IHC image of PSD95 (Phospho T19) staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16496, 1µg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  • PSD95 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to PSD95 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16496.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 105kDa, non specific band - 96kDa: We are unsure as to the identity of this extra band; PSD95

Anti-PSD95 (phospho T19) antibody (ab16496) 使用論文

ab16496 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% gel)
Sample Mouse Tissue lysate - whole (mouse brain tissue)
Specification mouse brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2013

Thank you for contacting us.

Currently we only have one lot in stock of ab16496, which is GR91281-1. We are not planning on making a new lot until all10 vials of this lotin stockare sold.

We have not heard any complaints about this...

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I am sorry for the delay in getting back to you.

I can now confirm that we do not unfortunately have any antibodies directed towards the N-terminal region of PSD95 which shares high homology with the drosophila dlg-A protein.

This m...

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Thank you for getting back to me and I am sorry for the delay in getting back to you.

From the reference provided, it seems that the protein you wish to detect (dlgA) is produced from the gene dlgfrom which dlg1 isexpressed. The isoform E of ...

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Thank you for contacting us and sorry for the delay in getting back to you.

I have looked intowhether theantibody you areinterested in, theanti-PSD95 (phospho T19) antibody (ab16496) would be likely to beable todetect the Drosophila protein a...

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Thank you for contacting me yesterday and this morning.

I am sorry for the delay in getting back to you but as explained over the phone I am trying to ascertain the likelihood of the PSD95 antibody being able to detect the drosophila protein....

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Feb 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Spinal cord dorsal horn lysate)
Loading amount 15 µg
Specification Spinal cord dorsal horn lysate
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

投稿 Mar 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"