製品の概要

  • 製品名
    Anti-PSD95 (phospho S295) antibody
    PSD95 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PSD95 (phospho S295)
  • 特異性
    PSD95 has a predicted band size of 95kDa, however in western blot gels it tends to run at a larger band size (~105kDa), this is thought to be due to post-translational modification (possibly palmitoylation) of PSD95 proteins.
  • アプリケーション
    適用あり: IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse PSD95, phosphorylated at S295.

    (Peptide available as ab17222.)

  • ポジティブ・コントロール
    • mouse hippocampus

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/300.
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 95 kDa).

ターゲット情報

  • 機能
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 組織特異性
    Brain.
  • 配列類似性
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • ドメイン
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻訳後修飾
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 細胞内局在
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

画像

  • Lane 1 - Input lane, 25-50µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)

    Lane 2 - IP lane, ~500µg mouse forebrain brain lysate probed with ab16495 PSD95 (phospho S295) antibody (1µg/ml)

    The IP lane (2) shows an enrichment of PSD95 (phospho S295) at the expected band size (~105kDa).

  • Immunohistochemistical detection of PSD95 (phospho S295) using antibody ab16495 on PFA perfusion-fixed mouse brain sections (30 microns). Primary antibody ab16495 was diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000). The image show immunostaining obtained in the mouse cerebellum using this antibody using direct fluorescence. The antibody stained granular cerebellar cells. Image [A] shows the staining obtained using the antibody, whereas image [B] is the staining captured with the same setup of acquisition for the negative control (lack of primary antibody). The arrows show the location of purkinje cells.

    See Abreview

参考文献

This product has been referenced in:
  • Lo SC  et al. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control. J Neurosci 35:2118-32 (2015). Read more (PubMed: 25653368) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Mar 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (4–12% Bis–Tris NuPAGE gradient gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Spinal cord lysate)
Loading amount
30 µg
Specification
Spinal cord lysate
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

投稿 Aug 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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