製品の概要

  • 製品名Anti-PSD95 antibody [6G6-1C9]
    PSD95 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6G6-1C9] to PSD95
  • アプリケーション適用あり: WB, IHC-Fr, IHC-FoFr, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Zebrafish
    交差が予測される動物種: Cow非交差種: Human
  • 免疫原

    Purified recombinant rat PSD-95.

  • ポジティブ・コントロール
    • In Western Blot, this antibody gave a positive signal in rat and mouse forebrain and hippocampus tissue lysates.
  • 特記事項

    Due to failure of current lot in ICC/IF, we no longer guarantee this application. Please contact Abcam Scientific Support team at techical@abcam.com for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/3000. PubMed: 22539981
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 組織特異性Brain.
  • 配列類似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • ドメインThe PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻訳後修飾Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 細胞内局在Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 antibody [6G6-1C9] 画像

  • Lanes 1 - 6 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution
    Lanes 7 - 12 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution (18 hours at 4°C)

    Lane 1 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 2 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 3 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 4 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 5 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 7 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 8 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 9 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 10 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 11 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C

    Lysates/proteins at 30 µg per lane.

    Secondary
    Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    Image courtesy of an AbReview submitted by Grant Corbett

    See Abreview

  • ab2723 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS- 0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab2723 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 (which stains the axons and dendrytic spines) can be observed in red (Alexa fluro 568 secondary). Synaptophysin is observable in green (alexa 488 secondary) and colocalizes with PSD95 in dendritic spines. Nuclei are stained in blue with 1.43µM Hoechst.

    See Abreview

  • All lanes : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 5 µg/ml

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,110 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ab2723 staining PSD95 in Zebrafish retina (outer plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab2723 at 1/200 staining rat primary hippocampal neurons by ICC/IF. The cells were paraformaldehyde fixed and then permeabilized with 0.1% Triton X-100 before being stained with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat polyclonal antibody was used as the secondary.

    The antibody shows numerous puncta along the shafts.

    See Abreview



  • Predicted band size : 80 kDa

    This image is courtesy of an anonymous Abreview

    All lanes : PSD95 antibody (ab2723) at 1/2000 dilution (in blocking buffer for 16 hours at 4°C) + whole tissue lysate of Mouse hippocampus at 10µg

    Secondary
    An HRP-conjugated Horse anti-mouse IgG polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions

    Exposure time : 30 seconds

    Blocking Step : 5% Milk for 1 hour at 25°C

    See Abreview

  • IHC-FoFr staining of PSD95 on rat hippocampus section using ab2723 (1:3000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-PSD95 antibody [6G6-1C9] (ab2723) 使用論文

This product has been referenced in:
  • Liu ZH  et al. Early developmental bisphenol-A exposure sex-independently impairs spatial memory by remodeling hippocampal dendritic architecture and synaptic transmission in rats. Sci Rep 6:32492 (2016). ICC/IF ; Rat . Read more (PubMed: 27578147) »
  • Zhao X  et al. Caspase-2 cleavage of tau reversibly impairs memory. Nat Med N/A:N/A (2016). WB ; Mouse . Read more (PubMed: 27723722) »

See all 37 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (retina, outer plexiform layer)
Specification retina, outer plexiform layer
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

投稿 Oct 02 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8% in TGS)
Sample Mouse Tissue lysate - whole (Hippocampus and Liver)
Specification Hippocampus and Liver
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Grant Corbett

Verified customer

投稿 Jul 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Tissue lysate - whole (mouse brain tissue)
Specification mouse brain tissue
Total protein in input 200 µg
Username

Abcam user community

Verified customer

投稿 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (mouse hippocampus and cerebellum)
Specification mouse hippocampus and cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jul 04 2012

We do not know how PSD95 expression would be affected if toxicity is increased in cells. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their s...

Read More

Thank you for contacting Abcam.

In mouse neurons an alternative to the smaller housekeeping proteins such as beta-actin, or GAPDH commonly used as loading control would be to use synaptic markers such as PSD95 or Piccolo.

In weste...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Hippocampal neurons)
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Specification Rat Hippocampal neurons
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Apr 12 2012

Thank you for contacting us.

To our knowledge, the epitope this clone recognizes has not been mapped. The immunogen was full-length rat PSD95.

If you do decide to try this antibody, we would of course be very interested in the res...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Brain Homogenate)
Loading amount 50 µg
Specification Brain Homogenate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 14 2012

1-10 of 14 Abreviews or Q&A

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