製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PSD93
  • 特異性
    Detects Post Synaptic Density 93 (PSD 93) from rat tissues. This antibody does not detect other synapse-associated protein family members.
  • アプリケーション
    適用あり: IHC-Fr, WB, ICC/IFmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Rat PSD93 aa 352-366.
    Sequence:

    NKLCDKPASPRHYSP


    (Peptide available as ab5840)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 5 µg/ml.
WB Use a concentration of 3 µg/ml. Detects a band of approximately 110 kDa.Can be blocked with PSD93 peptide (ab5840).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.
  • 配列類似性
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • ドメイン
    An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27.
  • 翻訳後修飾
    Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.
  • 細胞内局在
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Channel associated protein of synapse 110 antibody
    • Channel associated protein of synapses 110kD antibody
    • Channel-associated protein of synapse-110 antibody
    • Chapsyn 110 antibody
    • Chapsyn-110 antibody
    • Chapsyn110 antibody
    • Discs, large homolog 2 (Drosophila) antibody
    • Disks large homolog 2 antibody
    • DKFZp781D1854 antibody
    • DKFZp781E0954 antibody
    • Dlg 2 antibody
    • dlg2 antibody
    • DLG2_HUMAN antibody
    • Dlgh 2 antibody
    • Dlgh2 antibody
    • FLJ37266 antibody
    • Gm1197 antibody
    • MGC131811 antibody
    • Postsynaptic density protein PSD 93 antibody
    • Postsynaptic density protein PSD-93 antibody
    • Postsynaptic density protein PSD93 antibody
    • PSD 93 antibody
    • PSD93 antibody
    see all

画像

  • Immunolocalization of PSD-93 in rat cerebellum using ab2930.

  •  Western blot of PSD-93 on rat brain extract using ab2930.

  • ICC/IF image of ab2930 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2930, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

See all 3 Publications for this product

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