製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PSD3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: TSSNVEAAKR LAKRLYQLDR FKRSDVAKHL GKNNEFSKLV AEEYLKFFDF, corresponding to internal sequence amino acids 577-626 of Human PSD3

  • ポジティブ・コントロール
    • A204 cell lysate, human skeletal muscle tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性
    PSD3 is a guanine nucleotide exchange factor (GEF) specific for ARF6-like protein. The gene is potential tumour suppressor or antagonising gene candidate for ovarian carcinoma.
  • 細胞内局在
    Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity).
  • 参照データベース
  • 別名
    • ADP ribosylation factor guanine nucleotide factor 6 antibody
    • DKFZp761K1423 antibody
    • EFA 6R antibody
    • EFA6R antibody
    • Epididymis tissue protein Li 20mP antibody
    • Exchange factor for ADP ribosylation factor guanine nucleotide factor 6 antibody
    • HCA 67 antibody
    • HCA67 antibody
    • Hepatocellular carcinoma associated antigen 67 antibody
    • KIAA0942 antibody
    • PH and SEC7 domain containing protein 3 antibody
    • Pleckstrin and Sec7 domain containing 3 antibody
    • Pleckstrin and Sec7 domain containing protein 3 antibody
    • Pleckstrin homology and SEC7 domain containing protein 3 antibody
    • PSD 3 antibody
    • PSD3 protein antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling PSD3 with ab62194 at 4-8µg/ml. Arrows inidcate positively labelled skeletal muscle cells. Magnification: 400X.

  • Anti-PSD3 antibody (ab62194) at 2.5 µg/ml + A204 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

参考文献

ab62194 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry. The protocols used was as below. 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethanol for 1 minute each. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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