The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 (for cyanobacteria) - 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight of precursor: 39 kDa).
ab65579 may react in Synechocystis only with PsbA2/3 but not with PsbA1.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
psbA, also known as D1, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. It reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.