製品の概要

  • 製品名Anti-PRUNE antibody
    PRUNE 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PRUNE
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length human PRUNE protein (NP_067045.1).

  • ポジティブ・コントロール
    • WB: Raw 264.7 cell lysate, NIH/3T3 cell lysate, and PRUNE transfected 293T cell lysate ICC/IF: HeLa cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
  • 組織特異性Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
  • 配列類似性Belongs to the PPase class C family. Prune subfamily.
  • 細胞内局在Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DRES-17 antibody
    • DRES17 antibody
    • Drosophila-related expressed sequence 17 antibody
    • hPrune antibody
    • HTcD37 antibody
    • Protein prune homolog antibody
    • PRUNE antibody
    • Prune homolog antibody
    • PRUNE like protein antibody
    • PRUNE_HUMAN antibody
    • TcD37 homolog antibody
    see all

Anti-PRUNE antibody 画像

  • Anti-PRUNE antibody (ab88613) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-PRUNE antibody (ab88613) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PRUNE antibody (ab88613) at 1 µg/ml

    Lane 1 : PRUNE transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab88613, at 10 µg/ml, staining PRUNE in HeLa cell by Immunofluorescence.

Anti-PRUNE antibody (ab88613) 使用論文

ab88613 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (canine progenitor epidermal keratinocytes (CPEK))
Permeabilization Yes - 0.3% Triton X-100
Specification canine progenitor epidermal keratinocytes (CPEK)
Blocking step BioGenex Power Block 10 mins, then 10% Normal Donkey Serum 20 minutes as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Jan 11 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM, 0.05% Tween 20, pH 6.0
Permeabilization Yes - 0.05% Tween 20
Specification Skin
Blocking step BioGenex Power Block 10 minutes, then 10% Normal Donkey Serum 20 minutes as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Formaldehyde
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Verified customer

投稿 Nov 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"