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Our Abpromise guarantee covers the use of ab76781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
ab76781 staining PRSS2 in Human primary motor cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 2 hours at 20°C; antigen retrieval was by heat mediation in tris EDTA, pH9.2. Samples were incubated with primary antibody (1/200 in 2% serum, 0.5% Triton X-100 in PBS) for 20 hours at 20°C. An undiluted Biotin-conjugated Sheep anti-mouse IgG polyclonal was used as the secondary antibody.
ab76781 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"