製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

ターゲット情報

  • 機能
    Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer.
  • 配列類似性
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are 2 isoforms produced by alternative splicing.
  • 別名
    • AGOTC antibody
    • Homeobox protein PHOX1 antibody
    • Paired mesoderm homeo box 1 antibody
    • Paired mesoderm homeobox 1 antibody
    • paired mesoderm homeobox 1 isoform pmx-1b antibody
    • Paired mesoderm homeobox protein 1 antibody
    • Paired related homeobox 1 antibody
    • Paired related homeobox protein 1 antibody
    • Paired-related homeobox protein 1 antibody
    • PHOX 1 antibody
    • PHOX1 1, 2 antibody
    • PHOX1 antibody
    • PMX 1 antibody
    • PMX1 antibody
    • PRRX 1 antibody
    • Prrx1 antibody
    • PRRX1_HUMAN antibody
    • PRX 1 antibody
    • PRX-1 antibody
    • PRX1 antibody
    see all

画像

  • All lanes : Anti-PRRX1 antibody (ab67631) at 1/500 dilution

    Lane 1 : PRRX1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa

プロトコール

参考文献

ab67631 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Human HCC cell line and monocytes)
Specification
Human HCC cell line and monocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Shanshan Wan

Verified customer

投稿 Sep 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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