製品の概要

  • 製品名
    Anti-PRPK antibody
  • 製品の詳細
    Rabbit polyclonal to PRPK
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH, selected from the sequence of Human PRPK within amino acids 200-253.

  • ポジティブ・コントロール
    • Mouse thymus tissue lysate, Human breast carcinoma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
  • 組織特異性
    Highly expressed in testis. Weakly expressed in heart kidney and spleen.
  • 配列類似性
    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C20orf64 antibody
    • Nori 2 antibody
    • Nori 2p antibody
    • Nori-2 antibody
    • p53 related protein kinase antibody
    • p53-related protein kinase antibody
    • PRPK_HUMAN antibody
    • TP53 regulating kinase antibody
    • TP53-regulating kinase antibody
    • TP53RK antibody
    see all

画像

  • Anti-PRPK antibody (ab37606) at 1/100 dilution + Mouse thymus tissue lysate

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 15-20 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ab37606, at 1/50 dilution, staining PRPK in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37606 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

参考文献

This product has been referenced in:
  • Villarroel-Campos D  et al. Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. J Neurosci 36:7298-313 (2016). Read more (PubMed: 27383602) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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