製品の概要

  • 製品名Anti-PRPF31 antibody
    PRPF31 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PRPF31
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    KELGNSLDKCKNNEN

    , corresponding to internal sequence amino acids 140-154 of Human PRPF31

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab58588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報Peptide ELISA: antibody detection limit dilution 1:128,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

    An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.
    • 組織特異性Ubiquitously expressed.
    • 関連疾患Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.
    • 配列類似性Belongs to the PRP31 family.
      Contains 1 Nop domain.
    • ドメインInteracts with the snRNP via the Nop domain.
      The coiled coil domain is formed by two non-contiguous helices.
    • 細胞内局在Nucleus speckle. Nucleus > Cajal body. Predominantly found in speckles and in Cajal bodies.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp566J153 antibody
      • hPrp 31 antibody
      • hPrp31 antibody
      • NY BR 99 antibody
      • Pre mRNA processing factor 31 antibody
      • Pre mRNA processing factor 31 homolog (yeast) antibody
      • Pre mRNA processing factor 31 homolog antibody
      • Pre-mRNA-processing factor 31 antibody
      • Precursor mRNA-processing factor 31, S. cerevisiae, homolog of antibody
      • Protein 61K antibody
      • PRP 31 antibody
      • PRP31 antibody
      • PRP31 pre mRNA processing factor 31 homolog (yeast) antibody
      • PRP31 pre mRNA processing factor 31 homolog antibody
      • PRP31_HUMAN antibody
      • PRPF 31 antibody
      • prpf31 antibody
      • RP 11 antibody
      • RP11 antibody
      • Serologically defined breast cancer antigen NY BR 99 antibody
      • Serologically defined breast cancer antigen NY-BR-99 antibody
      • SNRNP61 antibody
      • U4/U6 small nuclear ribonucleoprotein Prp31 antibody
      • U4/U6 snRNP 61 kDa protein antibody
      see all

    Anti-PRPF31 antibody 画像

    • All lanes : Anti-PRPF31 antibody (ab58588) at 1 µg/ml

      Lane 1 : HeLa cell lysate (35µg protein in RIPA buffer) without blocking with the immunizing peptide
      Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


      Predicted band size : 55 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Anti-PRPF31 antibody (ab58588) 使用論文

    ab58588 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab58588.
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