製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PROX1
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Human PROX1 (C terminal). (Homeo domain and Prospero domain)
    Database link: Q92786

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/750 - 1/1000.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • 組織特異性
    Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Belongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • ドメイン
    The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • 細胞内局在
    Nucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • 別名
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Anti-PROX1 antibody 画像

  • Immunohistochemistry (Frozen sections) analysis of mouse embryo (day 13.5) tissue sections labelling PROX1 (red) with ab101851. Green - CD31, lv - large vessel, arrows - blood vessels, sg - sympathetic ganglion.

Anti-PROX1 antibody (ab101851) 使用論文

This product has been referenced in:
  • Hou H  et al. BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus. Stem Cell Reports 9:217-230 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28669600) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain section / Hippocampus)
Antigen retrieval step
None
Permeabilization
Yes - 3 x 5mn RT PBST
Specification
Brain section / Hippocampus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Yacine Tensaouti

Verified customer

投稿 Jul 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2017

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Reduced Denaturing (4-12)
Loading amount
40 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2017

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.1% Troton X-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 12 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3 % Troton X 100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 19 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (h9 cell line upon differentiation)
Specification
h9 cell line upon differentiation
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録