製品の概要

  • 製品名
    Anti-PROX1 antibody [5G10]
    PROX1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5G10] to PROX1
  • 特異性
    Specific to PROX1
  • アプリケーション
    適用あり: IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Recombinant Human PROX1 protein.

  • ポジティブ・コントロール
    • Rat denate gyrus tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000.

ターゲット情報

  • 機能
    Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • 組織特異性
    Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Belongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • ドメイン
    The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • 細胞内局在
    Nucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • 別名
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Anti-PROX1 antibody [5G10] 画像

  • ab92825, at a 1/1000 dilution in 0.1% BSA with 0.25% triton-X-100 in 0.1 M tris-saline, staining PROX1 in rat dentate gyrus tissue by immunohistochemistry (perfusion). The tissue was fixed via transcaridal perfusion with 2% paraformaldehyde and 3.75% acrolein in 0.1 M phosphate buffer (pH 7.4). Detection: DAB staining (6 minutes)

プロトコール

Anti-PROX1 antibody [5G10] (ab92825) 使用論文

ab92825 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.1% Troton X-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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投稿 Apr 13 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
40 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Epithelial)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
Epithelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
40 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Jan 26 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
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投稿 Dec 09 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Sample
Mouse Cell (mouse lens tissue)
Specification
mouse lens tissue
Permeabilization
Yes - 0.25%triton for 12mins
Fixative
Formaldehyde
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投稿 Jul 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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