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This antibody reacts with bacteria in suspension and is therefore useful for detection of Proteus spp. in bacterial suspensions. Various systems can be used with this antibody to detect the presence of Proteus spp.
Our Abpromise guarantee covers the use of ab8469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.
Detection by luminescence, polymerase chain reaction or culture. Magnisort-M particles (DuPont) are diluted 25 times in PBS (+ 1% gelatin). 50 µl is transferred into a microtiterplate well. Add 50-75 µl antibody solution, incubate shaking 15 min. at room temperature. Magnetize plate and remove supernatant. Add 75 µl bacteria suspension and incubate shaking 15 min. at room temperature. Magnetize plate and wash beads three times with PBS-gelatin. Resuspend beads in appropriate volume and prepare for specific detection.
ab8469 has not yet been referenced specifically in any publications.