Anti-Protein Z 抗体 (ab85317)
Key features and details
- Rabbit polyclonal to Protein Z
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Protein Z antibody
Protein Z 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Protein Z -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Protein Z aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129946) -
ポジティブ・コントロール
- This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Heart; Human Spleen; Human Bone Tumour; Human Bone Marrow. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85317の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa). |
ターゲット情報
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機能
Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. -
組織特異性
Plasma. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 8858 Human
- Omim: 176895 Human
- SwissProt: P22891 Human
- Unigene: 1011 Human
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別名
- Protein Z, vitamin K dependent plasma glycoprotein antibody
- PROZ antibody
- PROZ_HUMAN antibody
see all
画像
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All lanes : Anti-Protein Z antibody (ab85317) at 1 µg/ml (Milk Blocking - 5%)
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 42 kDa could potentially be a cleaved form of Protein Z due to the presemce of a 23 amino acid signal peptide. -
ICC/IF image of ab85317 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85317 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab85317 は論文での使用が確認できていません。