Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) 抗体 (ab27850)

製品の概要

  • 製品名Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody
    Protein phosphatase 1 inhibitor subunit 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Protein phosphatase 1 inhibitor subunit 2 (phospho T72)
  • 特異性ab27850 recognises Inhibitor-2.
  • アプリケーション適用あり: WB, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide (Human) - derived from the region that contains threonine 72.

  • ポジティブ・コントロール
    • HeLa or RAW 264.7 cells treated with nocodazole [200ng/ml] for 16 hours.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, 1.0 mg/mL BSA (IgG, protease free), Dulbecco's PBS [without magnesium and calcium ions]. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Inhibitor-2. The final product is generated by affinity chromatography using an Inhibitor-2 -derived peptide that is phosphorylated at threonine 72.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab27850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 23 kDa.
ICC 1/100.

ターゲット情報

  • 機能Inhibitor of protein-phosphatase 1.
  • 配列類似性Belongs to the protein phosphatase inhibitor 2 family.
  • 翻訳後修飾Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IPP 2 antibody
    • IPP-2 antibody
    • IPP2 antibody
    • IPP2_HUMAN antibody
    • MGC87148 antibody
    • Phosphatase inhibitor 2 antibody
    • Phosphoprotein phosphatase antibody
    • Ppp1r2 antibody
    • PPP1R2 protein antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 2 antibody
    • Protein phosphatase 1 regulatory subunit 2 antibody
    • Protein phosphatase inhibitor 2 antibody
    see all

Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody 画像

  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution

    Lane 1 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with NO added peptide
    Lane 2 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with non-phosphopeptide corresponding to the immunogen
    Lane 3 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with generic phosphothreonine containing peptide
    Lane 4 : Extracts of RAW 264-7 cells treated with 200ng/ml nocodazole with phosphopeptide immunogen

    Secondary
    F(ab')2 ant-rabbit IgG HRP conjugate
    Developed using the ECL technique

    Predicted band size : 23 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) at 1/1000 dilution

    Lane 1 : HeLa cell extract with NO nocodazole
    Lane 2 : HeLa cell extract with 200ng/ml nocodazole for 16 hours
    Lane 3 : RAW 264.7 cell extract with NO nocodazole
    Lane 4 : RAW 264.7 cell extract with 200ng/ml nocodazole for 16 hours
    Lane 5 : HeLa cell extract with an antibody recognizing total human IPP2 protein
    Lane 6 : HeLa cell extract with an antibody recognizing total human IPP2 protein

    Secondary
    F(ab')2 ant-rabbit IgG HRP conjugate
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 44/46 kDa (possible dimer),46 kDa,75 kDa. We are unsure as to the identity of these extra bands.

Anti-Protein phosphatase 1 inhibitor subunit 2 (phospho T72) antibody (ab27850) 使用論文

ab27850 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab27850.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"