Anti-Protein L 抗体 (HRP) (ab63506)


  • 製品名Anti-Protein L antibody (HRP)
    Protein L 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Protein L (HRP)
  • 標識HRP
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    Reacts with bacteria.
  • 免疫原

    Purified recombinant Protein L.




Our Abpromise guarantee covers the use of ab63506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent dilution.


  • 関連性Protein L is a 36,000 dalton immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains - about 65% of human immunoglobulins carry kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.
  • 細胞内局在Cell surface
  • 別名
    • ProteinL antibody
    • RPL antibody

Anti-Protein L antibody (HRP) (ab63506) 使用論文

ab63506 has not yet been referenced specifically in any publications.

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Thank you for your response.

A lysate ofPeptostreptococcus magnus,purified Protein L protein or recombinant Protein Lwould all be ideal positive controls for use withab63506.

Fora positive control to use with our anti GBA antibody...

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When choosing a loading control it is best practice to choose housekeeping genes which will be present in your sample type. For example, in whole cell preparations beta Actin or GAPDH will be appropriate while ...

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Currently, we have 4 antibodies against Protein L but none of them have been tested in immunoprecipitation:

ab63503, ab63504, ab63505, ab63506

If your customer would like to test any of these an...

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