製品の概要

  • 製品名
    Anti-Protein Kinase D2 (phospho S876) antibody
    Protein Kinase D2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Protein Kinase D2 (phospho S876)
  • 特異性
    Detects endogenous levels of PKD2 only when phosphorylated at serine 876
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PKD2 around the phosphorylation site of serine 876 (R-I-SP-V-L).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA 1/60000.

ターゲット情報

  • 機能
    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • 細胞内局在
    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

画像

  • All lanes : Anti-Protein Kinase D2 (phospho S876) antibody (ab59417) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins)
    Lane 2 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) with blocking phosphopeptide


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Sinnett-Smith J  et al. Protein Kinase D1 Mediates Stimulation of DNA Synthesis and Proliferation in Intestinal Epithelial IEC-18 Cells and in Mouse Intestinal Crypts. J Biol Chem 286:511-20 (2011). WB ; Rat . Read more (PubMed: 21051537) »

See 1 Publication for this product

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