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RabMAb

Anti-Protein Kinase D2 抗体 [EP1495Y] (ab51250)

製品の概要

  • 製品名
    Anti-Protein Kinase D2 antibody [EP1495Y]
    Protein Kinase D2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1495Y] to Protein Kinase D2
  • アプリケーション
    適用あり: WB, Flow Cyt, IHC-P, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • HeLa cell lysate or human breast carcinoma tissue. IF/ICC: HeLa cell line
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP1495Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
    • 組織特異性
      Widely expressed.
    • 配列類似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylation of Ser-876 correlates with the activation status of the kinase.
      Ser-706 is probably phosphorylated by PKC.
    • 細胞内局在
      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    画像



    • Predicted band size : 97 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Protein Kinase D2 knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51250 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51250 was shown to recognize Protein Kinase D2 when Protein Kinase D2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Protein Kinase D2 knockout samples were subjected to SDS-PAGE. Ab51250 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-Protein Kinase D2 antibody [EP1495Y] (ab51250) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 97 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • Ab51250 (1:100) staining human Protein Kinase D2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • ICC/IF image of ab51250 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51250, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    This product has been referenced in:
    • Bhavanasi D  et al. Protein kinase Cd mediates the activation of protein kinase D2 in platelets. Biochem Pharmacol 82:720-7 (2011). WB ; Human, Mouse . Read more (PubMed: 21736870) »
    • Azoitei N  et al. Protein kinase D2 is a novel regulator of glioblastoma growth and tumor formation. Neuro Oncol 13:710-24 (2011). WB, IHC-P ; Human, Chicken . Read more (PubMed: 21727210) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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