製品の概要

  • 製品名Anti-Protein Kinase D2 antibody
    Protein Kinase D2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Protein Kinase D2
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Human protein kinase D2.

  • 特記事項


    This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 105 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • 細胞内局在Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

Anti-Protein Kinase D2 antibody 画像

  • ab7281 staining Protein Kinase D2 in human BON cells (derived from a human pancreatic carcinoid tumor) by Immunocytochemistry/ Immunofluorescence.
    BON cells were grown on glass coverslips in 24-well plates. 2 or 3 days after seeding or transfection, cells were treated with vehicle (Me2SO) or PMA (100 nm) for 30 minutes. After treatment, cells were fixed with 4% paraformaldehyde for 20 minutes at 37 °C. After three washes with PBS, the cells were permeabilized with 0.3% Triton X-100 for 15 minutes at 37 °C and blocked with 1% bovine serum albumin/PBS for 10 minutes. The cells were incubated with the primary antibody diluted with 1% bovine serum albumin/PBS for 1 hour at room temperature. Cells were washed three times with PBS and incubated with Alexa-conjugated secondary antibody diluted by 1/500 in 1% bovine serum albumin/PBS.

    Double staining was performed using antibodies ab7281 and an anti-NT. Co-localization is shown by arrows in the merged images.
  • Anti-Protein Kinase D2 antibody (ab7281) at 1/500 dilution + whole cell lysate prepared from murine NIH3T3 cells at 25 µg

    Secondary
    Donkey anti-rabbit HRP at 1/5000 dilution
    Developed using the ECL technique

    Exposure time : 15 minutes

    Image courtesy of an anonymous Abreview.

  • ICC/IF image of ab7281 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Protein Kinase D2 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Protein Kinase D2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7281.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 101KDa: Protein Kinase D2

Anti-Protein Kinase D2 antibody (ab7281) 使用論文

This product has been referenced in:
  • Arun SN  et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . Read more (PubMed: 21132013) »
  • Li J  et al. PKD1, PKD2, and Their Substrate Kidins220 Regulate Neurotensin Secretion in the BON Human Endocrine Cell Line. J Biol Chem 283:2614-21 (2008). ICC/IF ; Human . Read more (PubMed: 18048355) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

This antibody was raised against the full length human Protein Kinase D2, plus it is a polyclonal, so the antibody will have multiple binding epitopes along the protein.

I've BLASTed the immunogen against...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount 25 µg
Specification NIH 3T3
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HMEC)
Loading amount 15 µg
Specification HMEC
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (placenta tissue-paraffin sections)
Specification placenta tissue-paraffin sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5%
Username

Dr. Daniel MacPhee

Verified customer

投稿 Jan 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"