製品の概要

  • 製品名Protein Block
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 特記事項PROTOCOL:
    Immunohistochemistry

    Incubate tissue section for 5 minutes at room temperature prior to application of the primary antibody. After incubation rinse once in buffer.
    ELISA
    Add Protein Block to microtiter well and incubate for 2-10 minutes prior to addition of sample. Rinse and continue procedure.
    Chemiluminescent Blot
    Protein Block has been reported to be an effective blocker for this technique with incubation times of one hour at room temperature or overnight at 4°C.


    ab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at +4°C.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.5% BSA, 0.5% Casein, PBS
  • Concentration information loading...
  • IHC 試薬 備考ab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.
  • 研究分野
  • 関連性Protein Block is used with immunolabeling techniques for the reduction of non-specific background staining.

アプリケーション

Our Abpromise guarantee covers the use of ab64226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Protein Block (ab64226) 使用論文

This product has been referenced in:
  • Skardal A  et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med 1:792-802 (2012). IHC-Fr . Read more (PubMed: 23197691) »
  • Takeda K  et al. Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Anticancer Res 31:263-70 (2011). Read more (PubMed: 21273608) »

See all 2 Publications for this product

Product Wall

ab64226 is the protein block used in ab80437. A link to ab64226 is below:

Thank you for contacting us.
The lab sent me the following information:
1. What is the compositions of the protein block? Does it contain BSA?
ANSWER: PBS solution, pH 7.6, with 0.5% BSA, 0.5% casein and less than 0.1% sodium azide
...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Most of our antibodies will remain stable for 7 days at room temperature. For ab28364, anti-CD31, it should still be viable and work as expected. As for the protein block, we have no information on how long it will remain viable at room temperature, bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"