Protein A/G/L Sepharose® (ab193264)
製品の概要
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製品名
Protein A/G/L Sepharose® -
製品の概要
Content:
Supplied as a 50% slurry in 0.02% thimerosal; >5 mg Protein A/G/L per mL of Sepharose® beads.
Features:
Protein A/G/L binds to all IgGs that bind to Protein A, Protein G, and Protein L, making it the ideal choice for purification of a wide range of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/mL of gel; high flow rate; low falling off of rProtein A/G/L; pH stability 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies.
- Immunoprecipitation.
Sepharose is a registered trademark of GE Healthcare
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 6541 Protein A/G/L Sepharose. 6541-25 is the same size as the 25 ml size of ab193264.
Protein A/G/L Sepharose® is prepared by covalently coupling recombinant Protein A/G/L (containing five Ig-binding regions from Protein L, five IgG binding domains from Protein A, and three Ig-binding regions from Protein G) to 6% cross-linked Sepharose® beads. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure maximum specific IgG binding. The coupling technique is optimized to give high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet gel.
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アプリケーション
適用あり: Purification, IPmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 5 ml 1 ml 25 ml 100 ml Protein A/G/L Sepharose® — — — — -
研究分野
関連製品
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab193264の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies. |
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IP |
Use at an assay dependent concentration.
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特記事項 |
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Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies. |
IP
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab193264 は論文での使用が確認できていません。