Anti-Protein A 抗体 [SPA-27] (Biotin) (ab49734)

製品の概要

  • 製品名Anti-Protein A antibody [SPA-27] (Biotin)
    Protein A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SPA-27] to Protein A (Biotin)
  • 標識Biotin
  • 特異性Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. The activity of this antibody is due to specific antigen antibody interaction with protein A; Protein A reacts minimally with the Fc portion of this mouse IgG1 antibody. The antibody will bind to protein A even in the presence of IgG.
  • アプリケーション適用あり: ELISA, Dot blotmore details
  • 種交差性
    交差種: Staphylococcus aureus
  • 免疫原

    Purified Protein A from Cowan I strain of Staphy­lococcus aureus.

  • 特記事項

    The immunoglobulin fraction of the ascites fluid is conjugated to epsilon amino caproyl biotin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Immunoglobulin Fraction of Mouse Ascites Fluid
  • ポリ/モノモノクローナル
  • クローン名SPA-27
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/80000.
Dot blot 1/5000.

ターゲット情報

  • 関連性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • 細胞内局在Secreted; cell wall.
  • 参照データベース
    • 別名
      • gG-binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody
      • Staphylococcal protein A antibody

    Anti-Protein A antibody [SPA-27] (Biotin) (ab49734) 使用論文

    ab49734 has not yet been referenced specifically in any publications.

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