製品の概要

  • 製品名Anti-Protein A antibody (Biotin)
    Protein A 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Protein A (Biotin)
  • 標識Biotin
  • アプリケーション適用あり: WB, ELISAmore details
  • 免疫原

    Recombinant full length protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin.
  • ポリ/モノポリクローナル
  • アイソタイプIgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.

ターゲット情報

  • 関連性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • 細胞内局在Secreted; cell wall.
  • 別名
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

Anti-Protein A antibody (Biotin) (ab18598) 使用論文

This product has been referenced in:
  • Gilbert FB  et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . Read more (PubMed: 23758654) »

See 1 Publication for this product

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The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"