製品の概要

  • 製品名Anti-Proteasome subunit beta type 4 antibody
    Proteasome subunit beta type 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Proteasome subunit beta type 4
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Proteasome subunit beta type 4 aa 1-265.
    Database link: P28070

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 µg/ml.
ICC/IF 1/500.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 配列類似性Belongs to the peptidase T1B family.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 26 kDa prosomal protein antibody
    • HN3 antibody
    • HsBPROS26 antibody
    • HSN3 antibody
    • Macropain beta chain antibody
    • Multicatalytic endopeptidase complex beta chain antibody
    • PROS-26 antibody
    • PROS26 antibody
    • Proteasome (prosome macropain) subunit beta type 4 antibody
    • Proteasome beta 4 subunit antibody
    • Proteasome beta chain antibody
    • Proteasome chain 3 antibody
    • Proteasome subunit beta type 4 antibody
    • Proteasome subunit beta type-4 antibody
    • Proteasome subunit HsN3 antibody
    • PSB4_HUMAN antibody
    • PSMB 4 antibody
    • PSMB4 antibody
    see all

Anti-Proteasome subunit beta type 4 antibody 画像

  • Proteasome subunit beta type 4 antibody (ab55628) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
  • Western blot against tagged recombinant protein immunogen using ab55628 Proteasome subunit beta type 4 antibody at 1ug/ml. Predicted band size of immunogen is 55 kDa
  • Overlay histogram showing HeLa cells stained with ab55628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55628, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Proteasome subunit beta type 4 antibody (ab55628) 使用論文

This product has been referenced in:
  • Thaker NG  et al. Identification of survival genes in human glioblastoma cells by small interfering RNA screening. Mol Pharmacol 76:1246-55 (2009). Read more (PubMed: 19783622) »
  • Catlow K  et al. Identification of a gastrin response element in the vesicular monoamine transporter type 2 promoter and requirement of 20 S proteasome subunits for transcriptional activity. J Biol Chem 282:17069-77 (2007). ICC/IF ; Human . Read more (PubMed: 17442673) »

See all 2 Publications for this product

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