Anti-Proteasome subunit beta type 2 抗体 [MCP165] (ab22650)

製品の概要

  • 製品名Anti-Proteasome subunit beta type 2 antibody [MCP165]
    Proteasome subunit beta type 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MCP165] to Proteasome subunit beta type 2
  • アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length protein corresponding to Human Proteasome subunit beta type 2.
    Database link: P49721

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 5mM Sodium Azide
    Constituents: 20mM Tris HCl, 100mM Sodium chloride, pH 8
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Partially purified by ion-exchange from cell culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名MCP165
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 28 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • 配列類似性Belongs to the peptidase T1B family.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all

    Anti-Proteasome subunit beta type 2 antibody [MCP165] 画像

    • Lane 1 : Protein Marker IV
      Lanes 2 - 7 : Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650) at 1/1000 dilution

      Lane 1 : Protein Marker IV
      Lane 2 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 3 : Protein extract from ciliary body of normal human donor at 2 µg
      Lane 4 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 5 : Protein extract from ciliary body of normal human donor at 2 µg
      Lane 6 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lane 7 : Protein extract from ciliary body of normal human donor at 2 µg

      Secondary
      Lanes 2 - 7 : HRP conjugated goat anti mouse antibody


      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an Abreview submitted by Dr Matthias Zenkel

      See Abreview

    • IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Proteasome subunit beta type 2 antibody [MCP165] (ab22650) 使用論文

    This product has been referenced in:
    • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . Read more (PubMed: 27633083) »
    • Ren H  et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). Read more (PubMed: 26508670) »

    See all 4 Publications for this product

    Product Wall


    I can confirm that this particular antibody is sourced externally from a collaborator. Unfortunately we are not able to release the immunogen sequence as they consider this proprietary information.

    They have confirmed that this antibody r...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (ciliary body)
    Loading amount 2 µg
    Specification ciliary body
    Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Dr. Matthias Zenkel

    Verified customer

    投稿 May 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"