Anti-Proteasome subunit alpha type 6 抗体 (ab97563)
Key features and details
- Rabbit polyclonal to Proteasome subunit alpha type 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Proteasome subunit alpha type 6 antibody
Proteasome subunit alpha type 6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Proteasome subunit alpha type 6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig, Zebrafish -
免疫原
Recombinant fragment corresponding to Human Proteasome subunit alpha type 6 aa 57-244.
Database link: P60900 -
ポジティブ・コントロール
- WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: A431 cells IHC-P: H1299 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97563の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5687 Human
- Entrez Gene: 26443 Mouse
- Entrez Gene: 29673 Rat
- Entrez Gene: 171585 Zebrafish
- Omim: 602855 Human
- SwissProt: P60900 Human
- SwissProt: Q9QUM9 Mouse
- SwissProt: P60901 Rat
see all -
別名
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
see all
画像
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Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution + mouse liver lysate at 50 µg
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
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All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution + rat liver lysate at 50 µg
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded mouse urinary bladder tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining Proteasome subunit alpha type 6 with ab97563 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97563 は 1 報の論文で使用されています。
- Piccinini AM et al. Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages. Sci Signal 9:ra86 (2016). WB . PubMed: 27577261