製品の概要

  • 製品名
    Anti-Proteasome 26S S3 antibody
    Proteasome 26S S3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Proteasome 26S S3
  • 特異性
    Detects proteasome 26S subunit S3.
  • アプリケーション
    適用あり: ICC, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human, Non human primates
    交差が予測される動物種: Cow, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 26S S3 aa 513-534.
    Sequence:

    EREQQDLEFAKEMAEDDDDSFP

  • ポジティブ・コントロール
    • HeLa cell extract.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).

For Western blot procedures it is necessary to transfer the protein to membrane at pH 11.

By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract.

ターゲット情報

  • 機能
    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • 配列類似性
    Belongs to the proteasome subunit S3 family.
    Contains 1 PCI domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 26S proteasome non ATPase regulatory subunit 3 antibody
    • 26S proteasome non-ATPase regulatory subunit 3 antibody
    • 26S proteasome regulatory subunit RPN3 antibody
    • 26S proteasome regulatory subunit S3 antibody
    • OTTHUMP00000164347 antibody
    • P58 antibody
    • Proteasome (prosome, macropain) 26S subunit non ATPase 3 antibody
    • Proteasome subunit p58 antibody
    • PSMD3 antibody
    • PSMD3_HUMAN antibody
    • RPN3 antibody
    • S3 antibody
    • Tissue specific transplantation antigen 2 antibody
    • TSTA2 antibody
    see all

画像

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of Proteasome 26S S3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3316 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

参考文献

ab3316 has not yet been referenced specifically in any publications.

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