製品の概要

  • 製品名Anti-Proteasome 20S LMP2 antibody
    Proteasome 20S LMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Proteasome 20S LMP2
  • 特異性Detects proteasome 20S LMP2.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 206-219.
    Sequence:

    RVILGNELPKFYDE


    Database link: P28065
    (Peptide available as ab4944)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーConstituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Can be blocked with Proteasome 20S LMP2 peptide (ab4944). (worked at 1/2000 (PMID 17114438))
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • 配列類似性Belongs to the peptidase T1B family.
  • 発生段階Highly expressed in immature dendritic cells (at protein level).
  • 翻訳後修飾Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

Anti-Proteasome 20S LMP2 antibody 画像

  • Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.

  • ab3328 (1µg/ml) staining Proteasome 20S LMP2 in human heart using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic cell compartments.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue (neurons in HIV encephalitis) sections labelling LMP2 with ab3328 at 1/1000. Immunofluorescence staining for LMP2 shows colocalization with the neuronal marker NeuN. LMP2 is present in granular deposits in the parikaryon. Scale bar = 10 μm.

Anti-Proteasome 20S LMP2 antibody (ab3328) 使用論文

This product has been referenced in:
  • Textor A  et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med 213:2333-2348 (2016). Read more (PubMed: 27697836) »
  • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). Read more (PubMed: 24971583) »

See all 16 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Human Cell lysate - whole cell (Lymphoma)
Specification Lymphoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. satyendra tripathi

Verified customer

投稿 Oct 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (dendritic cell)
Loading amount 1e+006 cells
Specification dendritic cell
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Jan 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Purified protein (heart and spleen)
Loading amount 1 µg
Specification heart and spleen
Gel Running Conditions Reduced Denaturing (12,5%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (B-LCL)
Loading amount 80000 cells
Specification B-LCL
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2007

Dear Anne All the information you have requested, including price, is available on the on line datasheets. I shall send them to you by e-mail also. You can order through the UK directly or through one of our distributors. Please use the ordering sectio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"