Anti-Proteasome 20S LMP2 抗体 (ab106824)

製品の概要

  • 製品名
    Anti-Proteasome 20S LMP2 antibody
    Proteasome 20S LMP2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Proteasome 20S LMP2
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Cow, Dog, Pig, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S LMP2 aa 104-113 (internal sequence) (Cysteine residue).
    Sequence:

    RNISYKYRED


    Database link: P28065

  • ポジティブ・コントロール
    • Human Thymus lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 23 kDa.

ターゲット情報

  • 機能
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
  • 配列類似性
    Belongs to the peptidase T1B family.
  • 発生段階
    Highly expressed in immature dendritic cells (at protein level).
  • 翻訳後修飾
    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta1i antibody
    • Large multifunctional peptidase 2 antibody
    • Large multifunctional protease 2 antibody
    • LMP 2 antibody
    • LMP2 antibody
    • Low molecular mass protein 2 antibody
    • Macropain chain 7 antibody
    • MGC70470 antibody
    • Multicatalytic endopeptidase complex chain 7 antibody
    • OTTHUMP00000062982 antibody
    • Proteasome (prosome macropain) subunit beta type 9 antibody
    • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody
    • Proteasome beta 9 subunit antibody
    • Proteasome catalytic subunit 1i antibody
    • Proteasome chain 7 antibody
    • Proteasome related gene 2 antibody
    • Proteasome subunit beta 6i antibody
    • Proteasome subunit beta type 9 antibody
    • Proteasome subunit beta type-9 antibody
    • Proteasome subunit beta-1i antibody
    • PSB9_HUMAN antibody
    • PSMB 9 antibody
    • PSMB6i antibody
    • PSMB9 antibody
    • Really interesting new gene 12 protein antibody
    • RING 12 antibody
    • RING12 antibody
    • RING12 protein antibody
    see all

画像

  • Anti-Proteasome 20S LMP2 antibody (ab106824) at 0.300000011920929 µg/ml + Rat Thymus lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 23 kDa

  • Anti-Proteasome 20S LMP2 antibody (ab106824) at 1 µg/ml + Human Thymus lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 23 kDa

プロトコール

参考文献

ab106824 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Tissue lysate - whole (Thymus)
Gel Running Conditions
Reduced Denaturing
Loading amount
45 µg
Treatment
Noninfected controls and virally infected samples
Specification
Thymus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Katie Knapek

Verified customer

投稿 Apr 18 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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