製品の概要

  • 製品名Anti-Proteasome 20S C2 antibody
    Proteasome 20S C2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Proteasome 20S C2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Zebrafish, Rainbow Trout, Leaf frog (Phyllomedusa), Orangutan, Zebra finch
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S C2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P25786
    (Peptide available as ab93073, ab93073)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HeLa whole cell; HepG2 whole cell; Jurkat whole cell; MCF7 whole cell; PC3 whole cell; HEK293 whole cell; human liver tissue; mouse heart tissue; rat heart tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

ターゲット情報

  • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
  • 配列類似性Belongs to the peptidase T1A family.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 30 kDa prosomal protein antibody
    • HC 2 antibody
    • HC2 antibody
    • Macropain subunit C2 antibody
    • Macropain subunit nu antibody
    • MGC14542 antibody
    • MGC14575 antibody
    • MGC14751 antibody
    • MGC1667 antibody
    • MGC21459 antibody
    • MGC22853 antibody
    • MGC23915 antibody
    • Multicatalytic endopeptidase complex subunit C2 antibody
    • NU antibody
    • PROS 30 antibody
    • PROS-30 antibody
    • PROS30 antibody
    • Proteasome (prosome macropain) subunit alpha type 1 antibody
    • Proteasome alpha 1 subunit antibody
    • Proteasome component C2 antibody
    • Proteasome nu chain antibody
    • Proteasome subunit alpha type 1 antibody
    • Proteasome subunit alpha type I antibody
    • Proteasome subunit alpha type-1 antibody
    • Proteasome subunit nu antibody
    • Protein P30 33K antibody
    • PSA1_HUMAN antibody
    • PSC 2 antibody
    • PSC2 antibody
    • PSMA 1 antibody
    • psmA1 antibody
    see all

Anti-Proteasome 20S C2 antibody 画像

  • All lanes : Anti-Proteasome 20S C2 antibody (ab82521) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : PC3 whole cell lysate (ab3954)
    Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa


    Exposure time : 3 minutes
  • All lanes : Anti-Proteasome 20S C2 antibody (ab82521) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-Proteasome 20S C2 antibody (ab82521) 使用論文

ab82521 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab82521.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"