The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Only tested in WB against the recombinant fragment used as immunogen
Use a concentration of 5 µg/ml.
機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens.
Yohannes E et al. Proteomic Signatures of Human Oral Epithelial Cells in HIV-Infected Subjects. PLoS One6:e27816 (2011).
Read more (PubMed: 22114700) »
O'hurley G et al. Evaluation of Zinc-alpha-2-Glycoprotein and Proteasome Subunit beta-Type 6 Expression in Prostate Cancer Using Tissue Microarray Technology. Appl Immunohistochem Mol Morphol18:512-517 (2010).
Read more (PubMed: 20661134) »