Anti-Proteasome 20S alpha 5 抗体 [EPR5833] (ab109533)

製品の概要

  • 製品名Anti-Proteasome 20S alpha 5 antibody [EPR5833]
    Proteasome 20S alpha 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5833] to Proteasome 20S alpha 5
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 5 aa 200-300 (C terminal).
    Database link: P28066

  • ポジティブ・コントロール
    • HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5833
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
    • 組織特異性Expressed in fetal brain (at protein level).
    • 配列類似性Belongs to the peptidase T1A family.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Macropain zeta chain antibody
      • Multicatalytic endopeptidase complex zeta chain antibody
      • Proteasome (prosome macropain) subunit alpha type 5 antibody
      • Proteasome alpha5 subunit antibody
      • Proteasome component 5 antibody
      • Proteasome subunit alpha type 5 antibody
      • Proteasome subunit alpha type-5 antibody
      • Proteasome subunit zeta antibody
      • Proteasome zeta chain antibody
      • PSA5_HUMAN antibody
      • PSC5 antibody
      • PSMA5 antibody
      • ZETA antibody
      see all

    Anti-Proteasome 20S alpha 5 antibody [EPR5833] 画像

    • All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5833] (ab109533) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa
    • ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Anti-Proteasome 20S alpha 5 antibody [EPR5833] (ab109533) 使用論文

    ab109533 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109533.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"