The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
ab63075 at 1/50 dilution, staining Proteasome 20S alpha 5 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. ab63075 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.