Anti-Proteasome 20S alpha 3 (phospho S250) 抗体 (ab79184)

製品の概要

  • 製品名Anti-Proteasome 20S alpha 3 (phospho S250) antibody
    Proteasome 20S alpha 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Proteasome 20S alpha 3 (phospho S250)
  • 特異性Detects endogenous levels of Proteasome 20S alpha 3 only when phosphorylated at serine 250.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S alpha 3 (phospho S250).
    Database link: P25788

  • ポジティブ・コントロール
    • Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab79184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA 1/5000.

ターゲット情報

  • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
  • 配列類似性Belongs to the peptidase T1A family.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HC8 antibody
    • Macropain subunit C8 antibody
    • MGC12306 antibody
    • MGC32631 antibody
    • Multicatalytic endopeptidase complex subunit C8 antibody
    • Proteasome (prosome macropain) subunit alpha type 3 antibody
    • Proteasome alpha 3 subunit antibody
    • Proteasome component C8 antibody
    • Proteasome subunit alpha type 3 antibody
    • Proteasome subunit alpha type-3 antibody
    • Proteasome subunit C8 antibody
    • PSA3_HUMAN antibody
    • PSC8 antibody
    • psmA3 antibody
    see all

Anti-Proteasome 20S alpha 3 (phospho S250) antibody 画像

  • All lanes : Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)
    Lane 2 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184) 使用論文

ab79184 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"