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RabMAb

Anti-Proteasome 20S alpha 3 抗体 [EPR5455] (ab109532)

製品の概要

  • 製品名
    Anti-Proteasome 20S alpha 3 antibody [EPR5455]
    Proteasome 20S alpha 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5455] to Proteasome 20S alpha 3
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 3 aa 50-150.
    Database link: P25788

  • ポジティブ・コントロール
    • A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR5455
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
    • 配列類似性
      Belongs to the peptidase T1A family.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HC8 antibody
      • Macropain subunit C8 antibody
      • MGC12306 antibody
      • MGC32631 antibody
      • Multicatalytic endopeptidase complex subunit C8 antibody
      • Proteasome (prosome macropain) subunit alpha type 3 antibody
      • Proteasome alpha 3 subunit antibody
      • Proteasome component C8 antibody
      • Proteasome subunit alpha type 3 antibody
      • Proteasome subunit alpha type-3 antibody
      • Proteasome subunit C8 antibody
      • PSA3_HUMAN antibody
      • PSC8 antibody
      • psmA3 antibody
      see all

    画像

    • All lanes : Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in HeLa cells by Immunofluorescence.

    参考文献

    This product has been referenced in:
    • Cirigliano SM  et al. The synthetic peptide CIGB-300 modulates CK2-dependent signaling pathways affecting the survival and chemoresistance of non-small cell lung cancer cell lines. Cancer Cell Int 17:42 (2017). ICC/IF ; Human . Read more (PubMed: 28373828) »
    • Li Y  et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Sci Rep 7:40698 (2017). WB ; Chicken . Read more (PubMed: 28079188) »

    See all 3 Publications for this product

    レビューと Q&A

    We do not have any data suggesting that the antibody has higher affinity for nuclear PSMA3 than for cytoplasmic PSMA3. The differences in staining intensity may be due to unequal distribution of the protein (more PSMA3 in the nucleus than in the cytopl...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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