Anti-PSMA3 抗体 [EPR5455] (ab109532)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5455] to PSMA3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PSMA3 antibody [EPR5455]
PSMA3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5455] to PSMA3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5455 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5684 Human
- Entrez Gene: 19167 Mouse
- Entrez Gene: 29670 Rat
- Entrez Gene: 408248 Rat
- Omim: 176843 Human
- SwissProt: P25788 Human
- SwissProt: O70435 Mouse
- SwissProt: P18422 Rat
see all -
別名
- HC8 antibody
- Macropain subunit C8 antibody
- MGC12306 antibody
see all
画像
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All lanes : Anti-PSMA3 antibody [EPR5455] (ab109532) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : JAR cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa -
ab109532, at 1/100 dilution, staining PSMA3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab109532, at 1/100 dilution, staining PSMA3 in HeLa cells by Immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab109532 は 7 報の論文で使用されています。
- Liu J et al. Immuno-oncological role of 20S proteasome alpha-subunit 3 in aggravating the progression of esophageal squamous cell carcinoma. Eur J Immunol 52:338-351 (2022). PubMed: 34755333
- Liu Q et al. The IFN-?-induced immunoproteasome is suppressed in highly pathogenic porcine reproductive and respiratory syndrome virus-infected alveolar macrophages. Vet Immunol Immunopathol 226:110069 (2020). PubMed: 32535163
- Takahashi M et al. USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience 9:433-450 (2018). PubMed: 30469013
- Moritz KE et al. The role of the immunoproteasome in interferon-?-mediated microglial activation. Sci Rep 7:9365 (2017). PubMed: 28839214
- Cirigliano SM et al. The synthetic peptide CIGB-300 modulates CK2-dependent signaling pathways affecting the survival and chemoresistance of non-small cell lung cancer cell lines. Cancer Cell Int 17:42 (2017). ICC/IF ; Human . PubMed: 28373828
- Li Y et al. Proteome Response of Chicken Embryo Fibroblast Cells to Recombinant H5N1 Avian Influenza Viruses with Different Neuraminidase Stalk Lengths. Sci Rep 7:40698 (2017). WB ; Chicken . PubMed: 28079188
- Geng S et al. Protein Interaction between Ameloblastin and Proteasome Subunit a Type 3 Can Facilitate Redistribution of Ameloblastin Domains within Forming Enamel. J Biol Chem 290:20661-73 (2015). PubMed: 26070558