Rabbit polyclonal to Proteasome 20S alpha 3
Mouse, Rat, Cow, Zebrafish
Recombinant fragment corresponding to Human Proteasome 20S alpha 3 aa 1-147.
Database link: P25788
MOLT4 whole cell lysate; U87 xenograft; Raji cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 28 kDa.
1/100 - 1/500.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
Belongs to the peptidase T1A family.
Information by UniProt
Macropain subunit C8 antibody
Western blot - Proteasome 20S alpha 3 antibody (ab96746)
Anti-Proteasome 20S alpha 3 antibody (ab96746) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 28 kDa 12% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S alpha 3 antibody (ab96746)
ab96746, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin-embedded U87 xenograft by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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