Anti-Proteasome 20S alpha 2 抗体 [EPR5453] (ab109525)


  • 製品名Anti-Proteasome 20S alpha 2 antibody [EPR5453]
    Proteasome 20S alpha 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 2 aa 50-150.
    Database link: P25787

  • ポジティブ・コントロール
    • HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR5453
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab109525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
    • 配列類似性Belongs to the peptidase T1A family.
    • 翻訳後修飾Phosphorylated on tyrosine residues; which may be important for nuclear import.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HC3 antibody
      • Macropain subunit C3 antibody
      • MU antibody
      • Multicatalytic endopeptidase complex subunit C3 antibody
      • PMSA2 antibody
      • Proteasome (prosome macropain) subunit alpha type 2 antibody
      • Proteasome alpha 2 subunit antibody
      • Proteasome component C3 antibody
      • Proteasome subunit alpha type 2 antibody
      • Proteasome subunit alpha type-2 antibody
      • Proteasome subunit HC3 antibody
      • PSA2_HUMAN antibody
      • PSC2 antibody
      • PSC3 antibody
      • PSMA 2 antibody
      • psmA2 antibody
      see all

    Anti-Proteasome 20S alpha 2 antibody [EPR5453] 画像

    • All lanes : Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 25 kDa
    • ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
    • ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2 in HeLa cells by Immunofluorescence.

    Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525) 使用論文

    This product has been referenced in:
    • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . Read more (PubMed: 24556681) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12 %)
    Sample Human Cell lysate - whole cell (HaCaT human immortalized keratinocyte cell line, 3)
    Specification HaCaT human immortalized keratinocyte cell line, 3
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Iva Doleckova

    Verified customer

    投稿 May 31 2014

    Thank you for your inquiry. Regarding ab109525, we have not actually measured non-IgG protein concentration. The only known non-IgG protein in our antibody is BSA (0.05%). Please contact us for the specific IgG concentration of the lot you received...

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