Proteasome 20S Activity Assay Kit (Fluorometric) (ab112154)

製品の概要

  • 製品名
    Proteasome 20S Activity Assay Kit (Fluorometric)
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプ
    Quantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    ab112154 Proteasome 20S Activity Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. ab112154 uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by the proteasome generates strongly green fluorescent R110 that is monitored fluorometrically at 520-530 nm with excitation at 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.

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  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Detection of Proteasome Activity in Jurkat cells. Jurkat cells were seeded on the same day at 500,000 cells/90 µL/well in a 96-well black wall/clear bottom plate. The cells were treated with or without 50 mM H2O2 for 30 minutes. The proteasome assay loading solution (100 µL/well) was added and incubated in a 5% CO2, 37 °C incubator for 3 hours. The fluorescence intensity was measured at Ex/Em = 490/525.

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レビューと Q&A

Merci pour votre requête. J'ai confirmé avec le laboratoire et je peux vous confirmer que les deux kits le ab107921 et le ab112154, fonctionnent sur du tissue de rat. Nous pouvons donc vous garantir ces kits aussi pour la mesure de l'activité de protea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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