Protease Activity Assay Kit (Fluorometric - Green) (ab112152)

製品の概要

  • 製品名Protease Activity Assay Kit (Fluorometric - Green)
    Protease キット 製品一覧
  • テスト
    1 x 500 test
  • サンプルの種類
    Plasma, Cell culture extracts, Other biological fluids, Whole Blood
  • アッセイタイプQuantitative
  • 全工程の試験時間
    1h 00m
  • 製品の概要

    Protease assays are widely used for the investigation of protease inhibitors and detection of protease activities. Monitoring various protease activities has become a routine task for many biological laboratories. Some proteases have been identified as good drug development targets.

    ab112152 Protease Activity Assay Kit is an ideal choice to perform routine assays for the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. ab112152 uses a fluorescent casein conjugate which is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). In the intact substrate, casein is heavily labeled with a green fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The assay can be performed in a convenient 96-well or 384-well microtiter plate format and readily adapted to automation. Its signal can be easily read with a fluorescence microplate reader at Ex/Em = 490/525 nm using FITC filter set.

    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab112152 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Protease Activity Assay Kit (Fluorometric - Green) 画像

    • Trypsin protease activity was analyzed by using ab112152. Protease substrate was incubated with 1 unit trypsin in the kit assay buffer. The control wells had protease substrate only (without trypsin). The fluorescence signal was measured starting from time 0 when trypsin was added using a microplate reader with a filter set of Ex/Em = 490/525 nm. Samples were done in triplicates.

    プロトコール

    Protease Activity Assay Kit (Fluorometric - Green) (ab112152) 使用論文

    ab112152 has not yet been referenced specifically in any publications.

    Product Wall

    The kit ab111750 should not be used with turbid or non-clear samples, but my contact at the lab did say that ab112152 or ab112153 might be ok to use. We have not tested them in this way so we can't say for sure. For all of these kits, the upper limit o...

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    We have only tested these kits with biological samples at this time, and we do not have information about their use with environmental samples. Could you tell me a little bit more about how these samples will be prepared? Do you know the approximate ra...

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    There is no need to measure the amount of protein. You only need to add a sample volume of 50 uL. For more details, see protocol booklet, Table 2, page 8.

    We would recommend using 0.5% Triton, incubate at room temperature for 15 min. Then, centrifuge the sample and use the supernatant for the assay. You can also use commercially available lysis buffer like, Mammalian cell lysis buffer  (see attached file).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"