Protease Activity Assay Kit (ab111750)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 500 pg/well
製品の概要
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製品名
Protease Activity Assay Kit
Protease キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Enzyme activity -
検出感度
< 500 pg/well -
全工程の試験時間
1h 00m -
製品の概要
Abcam's Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect < 500 pg/well Trypsin present in the sample
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K781 Protease Activity Fluorometric Assay Kit. K781-100 is the same size as the 100 test size of ab111750.
Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer I 1 x 25ml Fluoresence Standard VI 1 x 200µl Protease Positive Control 1 vial Protease Substrate 1 vial -
研究分野
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab111750 は 4 報の論文で使用されています。
- Kim JA et al. Bacillus velezensis TSA32-1 as a Promising Agent for Biocontrol of Plant Pathogenic Fungi. J Fungi (Basel) 8:N/A (2022). PubMed: 36294618
- Bertelsen AB et al. DisCoTune: versatile auxiliary plasmids for the production of disulphide-containing proteins and peptides in the E. coli T7 system. Microb Biotechnol 14:2566-2580 (2021). PubMed: 34405535
- Mohan S et al. Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration. Mol Neurodegener 16:51 (2021). PubMed: 34344440
- Alomrani S et al. Papain-like cysteine proteases are required for the regulation of photosynthetic gene expression and acclimation to high light stress. J Exp Bot 72:3441-3454 (2021). PubMed: 33686435