Anti-Prosurfactant Protein C 抗体 (ab90716)


  • 製品名Anti-Prosurfactant Protein C antibody
    Prosurfactant Protein C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prosurfactant Protein C
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Prosurfactant Protein C.

    (Peptide available as ab99881.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the Human and Mouse Lung Tissue lysates. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549. This antibody gave a positive result in IHC in the following FFPE tissue: human normal lung.



Our Abpromise guarantee covers the use of ab90716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
IP Use a concentration of 5 µg/ml.


  • 機能Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 関連疾患Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • 配列類似性Contains 1 BRICHOS domain.
  • 細胞内局在Secreted > extracellular space > surface film.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • pulmonary surfactant apoprotein-2 SP-C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL pVal antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SFTPC surfactant pulmonary associated protein C antibody
    • SMDP2 antibody
    • SP 5 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • SPC antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein c antibody
    • Surfactant proteolipid SPL-pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all

Anti-Prosurfactant Protein C antibody 画像

  • Prosurfactant Protein C was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to Prosurfactant Protein C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90716.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 23kDa; Prosurfactant Protein C

  • All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 25,28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ab90716 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90716 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of Prosurfactant Protein C staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90716, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Prosurfactant Protein C antibody (ab90716) 使用論文

This product has been referenced in:
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »
  • Cross DA  et al. AZD9291, an irreversible EGFR TKI, overcomes T790M-mediated resistance to EGFR inhibitors in lung cancer. Cancer Discov 4:1046-61 (2014). Read more (PubMed: 24893891) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lung)
Gel Running Conditions Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount 40 µg
Specification Lung
Blocking step Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Pete Serbedzija

Verified customer

投稿 May 25 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (lung)
Permeabilization Yes - 0.1% Triton X-100
Specification lung
Blocking step 10% Serum + 0.1% Triton X in PBS at room temperature as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Feb 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

投稿 Nov 25 2014

Thank you for contacting us.

These product are Immunogen affinity purified rabbit IgG polyclonal antibodies, not serum.

I also wanted to clarify a point about the 100% testing discount codes. Only one code may be used per vial purc...

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I am very happy to hear of your interest in testing these product. If you should decide to proceed I may generate a 100% tesdt code for each tested application of each product. So if you purchase both products and test each in IHC and in Flow Cytometry...

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Thank you for contacting us.

We have not tested these products in applications other than Western blot and cannot guarantee that these will work in IHC or in Flow Cytometry. If you are interested in trying these products in those assays on t...

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