Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Prosurfactant Protein B aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH). (Peptide available as ab116184)
This antibody gave a positive signal in both WI38 and A549 whole cell lysates.
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Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Western blot - Anti-Prosurfactant Protein B antibody (ab101804)
All lanes : Anti-Prosurfactant Protein B antibody (ab101804) at 1 µg/ml
Lane 1 :WI-38 whole cell lysate (ab3960) Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa Observed band size: 42 kDa Additional bands at: 39 kDa (possible cleavage fragment)
Exposure time: 16 minutes
The band observed at 39 kDa could potentially be a cleaved form of Prosurfactant Protein B due to the presence of a 24 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.